Click on this link to download the full high-definition interactive pdf for AJUR Volume 19 Issue 2 (September 2022) or https://doi.org/10.33697/ajur.2022.058

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Links to individual manuscripts, abstracts, and keywords are provided below.

p.3. Evaluating Driveway Cross Slopes and Social Equity in Cedar City, UT

Brock Anderson* & Jamie Spinney

Department of Geosciences, Southern Utah University, Cedar City, UT

https://doi.org/10.33697/ajur.2022.059

Student: brock20anderson00@gmail.com*

Mentor: jamie.spinney@suu.edu

ABSTRACT

The Americans with Disabilities Act (ADA) is the most comprehensive law governing accessibility, and it requires local governments to develop transition plans to become compliant. Among the key ADA requirements is a continuous unobstructed pedestrian circulation network that consists of a sidewalk that has a cross slope of no more than two degrees. The primary objective of this research was to evaluate whether driveway cross slopes in Cedar City were ADA compliant, so a digital level was used to measure a random sample of driveway cross slopes. A secondary objective was to determine whether there is evidence of social inequities in Cedar City’s pedestrian environment. The estimated value of each residential property (a proxy for income) was retrieved from Zillow® to evaluate the statistical relationship between incomes and driveway cross slopes. The results of this study indicate that there was no widespread evidence of social inequities. However, most driveway cross slopes (78.8 percent) were not ADA compliant and, thus, require retrofitting that should incorporate more widespread use of sidewalk buffer strips. The results also highlight priority areas for sidewalk improvements and can be used to inform a transition plan for sidewalk enhancements and funding.

KEYWORDS

Social Equity; ADA; Sidewalks; Advocacy Planning; Driveway Cross Slope; Walkability

p.11. Conversations About Mental Health and Well-being During the COVID-19 Pandemic: Why and How Restaurant Employees Talk With Each Other and Managers

Anamaria Tepordei* & Kirsten Foot

Department of Communication, University of Washington, Seattle, WA

https://doi.org/10.33697/ajur.2022.060

Student: anamaria.tepordei@outlook.com*
Mentor: kfoot@uw.edu

ABSTRACT
Restaurant employees in the United States have experienced unprecedented challenges to their mental health and well-being (MHW) during the COVID-19 pandemic, yet little is known about communication regarding MHW in the restaurant industry. Drawing on health, organizational, and interpersonal communication concepts, this exploratory, survey-based study probed whether, how, and why or why not restaurant employees in western Washington State conversed about MHW with one another and their managers during the winter of 2021. Key findings include that there are many reasons why some restaurant employees do not engage in conversations about MHW with other members of the workplace. However, when such conversations do occur, they are typically mutual, positive, and relationally-oriented—more so among coworkers than between employees and managers. Additionally, both coworkers and managers are sources of social support and resource exchange during these conversations, although the evidence is stronger among coworkers. Our findings contribute to the extant literature on mental health communication in the workplace and demonstrate the merit in more closely examining superior-subordinate and coworker communication about personal and sensitive topics, like MHW. Comparative analysis of employees’ MHW-related communication with coworkers versus managers revealed both similarities and differences that carry implications for managerial practice and future research.

KEYWORDS
Mental Health and Well-being (MHW); Disclosure; Superior-subordinate Communication; Peer Coworker; Social Exchange; Social Support; Interpersonal Communication Motives (ICM), Restaurant Employees

p. 33. Differential Expression of Hub Genes and Activation of p53 by Anti-cancer Compound Curaxin CBL0137

Tanvi Patel*, Rochelle Ratner, & Niharika Nath

Department of Biological & Chemical Sciences, New York Institute of Technology, New York, NY

https://doi.org/10.33697/ajur.2022.061

Students: tpatel60@nyit.edu*, rratne02@nyit.edu

Mentor: nnath@nyit.edu

ABSTRACT

Cancer is a global concern and there is a need for effective drugs. CBL0137 is a small water-soluble molecule and a new second-generation compound in the family of curaxins with potential anti-cancer activity. Curaxins in general, including CBL0137 intercalate into DNA, and act by targeting the histone chaperone ‘facilitates chromatin transcription’ (FACT) complex and have the potential to treat tumors by reducing the growth of cancer cells which is shown in a variety of cell lines and animal models. CBL0137 is found to activate the tumor suppressor gene p53. However, the mechanism of p53 activation is poorly understood. Utilizing bioinformatics analysis on available datasets of CBL0137 treated cancer cells of glioma, cervical and multiple myeloma, differentially expressed genes (DEGs) that may lead to the activation of p53 were examined. Three GEO datasets of cells treated with various concentrations of CBL0137 were analyzed, namely HSJD-DIPG007 (GSE153441), MM1.S (GSE117611) and HeLa S3 (GSE117611). The DEGs were identified based on p-values less than 0.05, logFC values greater than 1 and less than -1 and analyzed using GEO2R, Enrichr, and STRING, and data visualization was performed on Tableau. Compared to the controls, a total of 229, 1425, and 1005 genes were upregulated while 368, 2322, and 1673 genes were downregulated for HSJD-DIPG007, MM1.S and HeLa S3 datasets, respectively. Further collective analysis revealed a total of 38 common DEGs among the three datasets. Using Enrichr and STRING on these 38 DEGs, seven hub genes were obtained, SKP2, RGS16, CSRP2, CENPA, HJURP, DTL, and HEXIM1 with these possible mechanisms:  inhibition of AKT phosphorylation by upregulated genes RGS16 and CSRP2, p300-mediated acetylation of p53 via SKP2, inhibition of MDM2 by DTL downregulation and HEXIM1 upregulation, and inhibition of AURKB via CENPA and HJURP downregulation. This study analyzed the three datasets and highlighted how these identified hub genes may play a role in leading to p53 activation by CBL0137.

KEYWORDS

Curaxin; CBL0137; Differentially Expressed Genes; Cancer; p53; Glioblastoma; Cervical; Myeloma

p. 43. Cemetery Analysis of Whitewater, WI

David H. Nehlsen

Department of Political Science and Philosophy at the University of Green Bay, WI

https://doi.org/10.33697/ajur.2022.062

Students: nehldh03@uchicago.edu*

Mentor: weinscha@uwgb.edu

ABSTRACT

Procedures and customs surrounding funerals, burial, and grave marking vary widely by time period, location, culture, and religion, among many other factors. This project investigated the gravestone customs of cemeteries in Southern Wisconsin, USA. Utilizing a dataset of 500 gravestone samples collected from cemeteries in Whitewater, WI, the aim of this project was to objectively measure how people choose to remember their dead and how these practices have evolved over the years. Because of the decline in American religiosity in recent decades, the expectation of this project was to see the usage of religious symbols decline. Based upon this analysis, this does not appear to be the case. Practices such as the recording of one’s name and year of birth/death have remained constant; however, other customs of remembrance, such as the use of religious symbols and the recording of one’s date of marriage, have shifted dramatically over the years, reflecting the evolution of society and culture.

KEYWORDS

Cemetery; Symbols; Funeral; Thanatology; Death; Demography; Monuments; History

Click on this link to download the full high-definition interactive pdf for AJUR Volume 19 Issue 1 (June 2022) or https://doi.org/10.33697/ajur.2022.053

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Links to individual manuscripts, abstracts, and keywords are provided below.

p.3 Myrica cerifera, a Medicinal Plant of the Lumbee Tribe, has Antibacterial and Nematicidal Properties

Ashley Edwards, Kazhmiri Deberry, Hannah Mariani, Darian H. Taylor, Nicholas J. Cochran, Ana C. Barrios Sosa, Andrea Regan Scott, R. Thomas Williamson, Cornelia Tirla, Conner Sandefur, & Courtney Carroll Alexander

https://doi.org/10.33697/ajur.2022.054

ABSTRACT: Currently threatening the world of medicine is a growing number of antibiotic-resistant diseases. More specifically, bacteria and nematodes have gained resistance to many of the world’s leading antibiotics and nematicides, respectively, making infections more difficult to treat. Subsequently, these parasitic organisms are able to continue damaging crops and other living organisms like humans without strong interference. To help people and the environment, the development of new and novel antibiotics is vital. Previous research suggests that phytochemicals are a potential solution that will not only help inhibit bacterial growth but also reduce nematode survival. We hypothesized that Myrica cerifera, a plant often used by the Lumbee tribe to treat illness, possesses antibacterial and nematicidal properties. To answer our hypothesis, we began by collecting plant specimens to extract material for biological assays and to subsequently isolate and elucidate the structures of active components. The extract was evaluated for antibacterial properties with an agar diffusion assay and then nematicidal properties using Caenorhabditis elegans. M. cerifera extract was added onto an agar lawn at various doses, and the nematodes’ lifespans were scored. The findings of this study show that extracts of this plant, more commonly referred to as ‘wax myrtle’, do significantly decrease the lifespan of C. elegans and increase the zone of inhibition for Staphylococcus epidermidis and Staphylococcus aureus. In addition, two compounds were isolated and characterized through chemical extraction, chromatographic separation, and spectroscopic analysis. These compounds could potentially be used to treat bacterial and nematode infections.

KEYWORDS: Antibacterial; Antimicrobial; Caenorhabditis elegans; Plant extract; Myrica cerifera; Nematicidal; NMR; Phytochemical

Supplemental Data for https://doi.org/10.33697/ajur.2022.054

p.13 State Adoption of Cryptocurrency: a Case Study Analysis of Iran, Russia, and Venezuela

Rose Mahdavieh

https://doi.org/10.33697/ajur.2022.055

ABSTRACT: The emergence of digital currency is becoming prevalent in the age of globalization – specifically, cryptocurrencies, a subset of digital currency that encompass revolutionary technology. This study postulates that certain governments are more prone to adopting cryptocurrencies, especially those seeking to eschew international sanctions and protect corrupt practices. Three comparative case studies focus on countries (Iran, Russia, and Venezuela) that share attributes that result in adopting what has been called “native cryptocurrencies”: corruption, GDP level, economic volatility, and Western sanctions.

KEYWORDS: Cryptocurrency; Blockchain; Political Science; Law; Foreign Sanctions; Government; Iran; Russia; Venezuela

p23. Salinity Affects Wound Healing in Wild Common Bottlenose Dolphins (Tursiops truncatus)

Brianna Hurst & Dara N. Orbach

https://doi.org/10.33697/ajur.2022.056

ABSTRACT: Dolphins are often individually identified by unique naturally-acquired markings. Identification becomes difficult when markings heal, or new scars appear. As salt accelerates wound healing in many organisms, the diminishment of scars on common bottlenose dolphins (Tursiops truncatus) residing in varying natural salinities was determined. South Texas contains the only hypersaline lagoon in the USA, located adjacent to hyposaline waters, with genetically distinct populations of dolphins in the two environments. Photographs of dolphin dorsal fins were collected, and scar stability over time was determined and compared by measuring changes in the relative lengths and surfaces of scars. All scars on dolphins in the hypersaline lagoon completely diminished between three to six years, while scars on dolphins in the hyposaline bay ranged in the amount of fading between three to six years. Data from this case study indicate that high salinity may increase the healing speed of wounds on common bottlenose dolphins compared to low salinity, although a larger sample size is needed for robust statistical comparison. Scar diminishment is an important consideration in determining the temporal reliability of photo identification.

KEYWORDS: Bottlenose dolphin; Corpus Christi Bay; healing; hypersaline; Laguna Madre; photo-identification; salinity; scar

p.31 Validation of Accelerometer-Based Estimations of Energy Expenditure During High-Intensity Interval Training

Nicholas Remillard, Marisa Mulvey, Gregory Petrucci Jr, & John R Sirard

https://doi.org/10.33697/ajur.2022.057

ABSTRACT: Accelerometers are used to assess free-living physical activity (PA) and energy expenditure (EE). Energy expenditure estimation algorithms have been calibrated using steady-state exercise. However, most free-living PA is not steady-state. Objective: The purpose of this study was to discern the differences between criterion-measured and accelerometer-estimated EE (kCals) during a non-steady-state High-Intensity Interval Training (HIIT) session. Methods: Recreationally active adults (N=29, 18-30 years) completed one of two HIIT protocols. Each participant wore ActiGraph GT3X+ accelerometers on the right hip and non-dominant wrist while EE was measured using portable indirect calorimetry. Data analysis was conducted using custom R scripts and bias [95% CIs] to determine significant differences between indirect calorimetry and EE estimates using previously developed algorithms. Results: All accelerometer algorithms underestimated EE during recovery intervals (range; -4.31 to -6.55 kCals) and overestimated EE during work intervals (0.57 to 5.70 kcals). Over the whole HIIT session, only the Hildebrand wrist method was not significantly different from the criterion measured EE. Conclusion: Current ActiGraph EE estimations based on steady-state activities underestimate EE during recovery periods of treadmill HIIT sessions. Future studies should investigate accelerometer signals immediately after high-intensity bouts to more accurately predict EE of the subsequent recovery period.

KEYWORDS: ActiGraph; Accelerometer; HIIT; Indirect calorimetry; EPOC; Energy expenditure; Non-steady state; Calories

Click on this link to download the full high-definition interactive pdf for AJUR Volume 18 Issue 4 (March 2022) or https://doi.org/10.33697/ajur.2022.050

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Links to individual manuscripts, abstracts, and keywords are provided below.

p.3. The Effects of the NBA COVID Bubble on the NBA Playoffs: A Case Study for Home-Court Advantage
Michael Price & Jun Yan
https://doi.org/10.33697/ajur.2022.051
ABSTRACT: The 2020 NBA playoffs were played inside of a bubble at Disney World because of the COVID-19 pandemic. This meant that there were no fans in attendance, games were played on neutral courts and no traveling for teams. In theory, these conditions should remove home-court advantage from the games. This setting generated discussion and concern, as analysts and fans debated the possible effects it may have on the outcome of games. Home-court advantage has historically played an influential role in NBA playoff series outcomes. The 2020 playoffs provided a unique opportunity to study the effects of the bubble and home-court advantage by comparing the 2020 season with the seasons in the past. While many factors contribute to the outcome of games, points scored is the deciding factor of who wins. Thus, scoring is the primary focus of this study. The specific measures of interest are team scoring totals and team shooting percentage on two-pointers, three-pointers, and free throws. Comparing these measures for home teams and away teams in 2020 vs. 2017-2019 shows that the 2020 playoffs favored away teams more than usual, particularly with two-point shooting and total scoring.
KEYWORDS: NBA; NBA Covid; NBA Bubble; Homecourt Advantage

p.15. Internalizing Symptoms in Children Exposed to Adversity: Examining Associations with Resilience, Social Support, and Community Cohesion
Rachel Stobbe, Taylor Napier, Debra Bartelli, & Kathryn H. Howell
https://doi.org/10.33697/ajur.2022.052
ABSTRACT: Exposure to adverse childhood experiences (ACEs) contributes to increased rates of psychopathology in youth. Specific environmental factors have been linked to improved functioning following adversity, but few studies have taken a social-ecological approach to examine how resilience, social support, and community cohesion may be associated with internalizing problems (i.e., anxiety, depression) in young children. The current study included 49 children between the ages of 8 and 13 (Mage = 10.43, SD = 1.57; 55.1% male; 95.8% Black or African American) who were recruited from four community programs in the Midsouth, United States that serve families experiencing adversity. Regarding income, 77.3% of youth’s caregivers reported an annual household income under $15,000. Almost all children reported experiencing at least one ACE (92.6%). Two linear regression models were run to assess how resilience, social support, and community cohesion were related to youth’s depression and anxiety symptoms while controlling for ACEs and family income. The model examining depression was significant, (F (5, 48) = 4.16, p < .01, R2 = .33) with fewer reported ACEs (β = 1.55, p < .02) and higher resilience (β = -.73, p = .01) associated with lower depressive symptoms. The model assessing anxiety was not significant. Results indicate that personal resilience may be a key target for intervention in children exposed to ACEs as efforts to strengthen individual resources (e.g., self-efficacy, emotion regulation skills) could be linked to reduced psychopathology.
KEYWORDS: Adverse Childhood Experiences; Social Ecology; Resilience; Social Support; Community Cohesion; Internalizing Symptoms; Anxiety; Depression

Click on this link to download the full high-definition interactive pdf for AJUR Volume 18 Issue 3 (December 2021)

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Links to individual manuscripts, abstracts, and keywords are provided below.

p. 3. An Optimal Control Experiment for an SEIRS Epidemiological Model
Tanner Snyder & Ryan Nierman
Department of Mathematics and Computer Science, Madonna University, Livonia, MI
https://doi.org/10.33697/ajur.2021.047
ABSTRACT: This work studies an optimal control model for a discrete-time Susceptible/Exposed/Infective/Removed/Susceptible (SEIRS) deterministic epidemiological model with a finite time horizon and changing population. The model presented converts a continuous SEIRS model that would typically be solved using differential equations into a discrete model that can be solved using dynamic programming. The discrete approach more closely resembles real life situations, as the number of individuals in a population, the rate of vaccination to be applied, and the time steps are all discrete values. The model utilizes a previously developed algorithm and applies it to the presented SEIRS model. To demonstrate the applicability of the algorithm, a series of numerical results are presented for various parameter values.
KEYWORDS: Control; Cost; Discrete; Disease; Epidemiology; Minimization; Modeling; Optimality; SEIRS; Vaccination

p. 15. Factors Associated with Surgery Among South Asian American and Non-Hispanic White Women with Breast Cancer
Lydia Lo & Jaya M. Satagopan
Department of Industrial and Systems Engineering, School of Engineering, Rutgers University,New Brunswick, NJ
Department of Biostatistics and Epidemiology & Center for South Asian Quantitative Health and Education, School of Public Health, Rutgers University, New Brunswick, NJ
https://doi.org/10.33697/ajur.2021.048
ABSTRACT: South Asian American (SA) women are diagnosed with more aggressive breast cancer than non-Hispanic White (NHW) women. Understanding the factors associated with the types of surgery received by these women sheds light on disease management in these culturally distinct populations. We used data on age at diagnosis, stage, grade, estrogen and progesterone receptors, and surgery from 4,590 SA and 429,030 NHW breast cancer cases in the Surveillance, Epidemiology and End Results (SEER) program. We used logistic regression with surgery as the binary outcome (subcutaneous, total, or radical mastectomy (STRM) versus partial mastectomy, no, unknown or other (PNUM)) and included additive effects of all the variables and interactions of age, stage, grade, and estrogen and progesterone receptors with race/ethnicity. Type I error of 5% was used to assess statistical significance of the effects. SA were significantly more likely than NHW cases to receive STRM relative to PNUM surgery among women diagnosed at or after age 50 years and having localized stage disease (Odds Ratio (OR) = 1.27, 95% Confidence Interval (CI) = 1.06 – 1.52). Further, SA were significantly less likely than NHW cases to receive STRM relative to PNUM surgery among those diagnosed before age 50 years and having regional or distant stage disease (OR = 0.75, 95% CI = 0.59 – 0.95 for age at diagnosis < 40 years; OR = 0.77, 95% CI = 0.62 – 0.95 for age at diagnosis 40-49 years). The type of surgery received by SA and NHW women differ according to age at diagnosis and disease stage. 
KEYWORDS: Breast Cancer; Surgery; Cancer Health Equity; Disease Characteristics; South Asian American; Non-Hispanic White; Logistic Regression; Interaction

p. 25. The Effect of Perceived Uncertainty on Competitive Behavior
Duncan James Drewry & Zachary Reese
Weinberg Institute for Cognitive Science, University of Michigan, Ann Arbor, MI
Department of Psychology, University of Michigan, Ann Arbor, MI
https://doi.org/10.33697/ajur.2021.049
ABSTRACT: How do people behave in the face of uncertainty? Some studies suggest that even when they are unaware of how others will behave, people default to cooperative behavior; however, other research suggests that uncertainty leads to more competitive behavior. Little research has examined how individual differences moderate such behavioral decisions. This study proposes that a stable (dispositional) sense of justice may, ironically, lead to more competitive behavior. Specifically, people who score highly in belief in a just world, system justification, and religiosity, and low in ambiguity tolerance may be more inclined to compete rather than cooperate because they believe people who experience positive outcomes deserve those outcomes regardless of the means taken to achieve them. Across two studies, participants (N = 288) engaged in a prisoner’s dilemma game — a task where they must choose to compete or cooperate — and completed the aforementioned individual difference measures. Results show that people tended to cooperate, but those high in system justification and belief in a just world were more likely to compete. In other words, people with a strong sense of cosmic justice were likely to exhibit competitive behavior under uncertain conditions.
KEYWORDS: Ambiguity Tolerance; Competition; Cooperation; Just World Beliefs; Prisoner’s Dilemma; Prosocial Behavior; Religiosity; System Justification; Uncertainty

Click on this link to download the full high-definition interactive pdf for AJUR Volume 18 Issue 2 (September 2021)

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p.3. On Packing Thirteen Points in an Equilateral Triangle
Natalie Tedeschi
https://doi.org/10.33697/ajur.2021.042
ABSTRACT: The conversation of how to maximize the minimum distance between points – or, equivalently, pack congruent circles- in an equilateral triangle began by Oler in the 1960s. In a 1993 paper, Melissen proved the optimal placements of 4 through 12 points in an equilateral triangle using only partitions and direct applications of Dirichlet’s pigeon-hole principle. In the same paper, he proposed his conjectured optimal arrangements for 13, 14, 17, and 19 points in an equilateral triangle. In 1997, Payan proved Melissen’s conjecture for the arrangement of fourteen points; and, in September 2020, Joos proved Melissen’s conjecture for the optimal arrangement of thirteen points. These proofs completed the optimal arrangements of up to and including fifteen points in an equilateral triangle. Unlike Melissen’s proofs, however, Joos’s proof for the optimal arrangement of thirteen points in an equilateral triangle requires continuous functions and calculus. I propose that it is possible to continue Melissen’s line of reasoning, and complete an entirely discrete proof of Joos’s Theorem for the optimal arrangement of thirteen points in an equilateral triangle. In this paper, we make progress towards such a proof. We prove discretely that if either of two points is fixed, Joos’s Theorem optimally places the remaining twelve.
KEYWORDS: optimization; packing; equilateral triangle; distance; circles; points; thirteen; maximize

p.13. An Unbiased Mineral Compositional Analysis Technique for Circumstellar Disks
Yung Kipreos & Inseok Song
https://doi.org/10.33697/ajur.2021.043
ABSTRACT: A circumstellar disk that surrounds a star is composed of gas, dust, and rocky objects that are in orbit around it. Around infant stars, this disk can act as a source of material that can be used to form planetesimals, which can then accrete more material and form into planets. Studying the mineral composition of these disks can provide insight into the processes that created our solar system. The purpose of this paper is to analyze the mineral composition of these disks by using a newly created python package, Min-CaLM. This package determines the relative mineral abundance within a disk by using a linear regression technique called non-negative least square minimization. The circumstellar disks that are capable of undergoing compositional analysis must have a spectrum with both a detectable mid-infrared excess and prominent silicate features. From our sample, there are only eight debris disks that qualify to be candidates for the Min-CaLM program. The mineral compositions calculated by Min-CaLM are then compared to the Tholen asteroid classification scheme. HD 23514, HD 105234, HD 15407A, BD+20 307, HD 69830, and HD 172555 are found to have a compositions similar to that expected for C-type asteroids, TYC 9410-532-1 resembles the composition of S-type asteroids, and HD 100546 resembles D-type asteroids. Min-CaLM also calculates the mineral compositions of the comets Tempel 1 and Hale-Bopp, and they are used as a comparison between the material in our early solar system and the debris disk compositions.
KEYWORDS: Debris disk; Mineral; Composition; Analysis; Asteroid; Circumstellar; Spectroscopy; Python

p.29. Do Warmups Predict Pole Vault Competition Performance?
Alex Peskin
https://doi.org/10.33697/ajur.2021.044
ABSTRACT: The aim of this research was to determine the relationship between pole vault warmup and competition performance in a sample of 16 collegiate vaulters over 60 observations. Pole vault athletes are given time to warm up in the same area that the competition will take place. This prompted investigation into whether better warmup performance could indicate better familiarity with the performance environment, and whether this could translate to the competition. The number of warmup vaults taken was also considered. Participants were observed during multiple warmup periods and data was collected on warmup performance. The findings indicate a significant correlation between instances in which participants displayed their best warmup scores and their best competition performances, likewise with their worst. Also, participants who took more warmup vaults performed significantly better on average. Athletes and coaches should consider implementing warmup practices that emphasize familiarizing oneself with their performance environment.
KEYWORDS: Pole Vault; Track and Field; Warmups; Warmup Performance; Competition Performance; Performance Environment; Nested Task; Task Constraints

p.35. A Narrative Literature Review of the Psychological Hindrances Affecting Return to Sport After Injuries
Ashley Sweeney, Stephanie M. Swanberg, & Suzan Kamel-ElSayed
https://doi.org/10.33697/ajur.2021.045
ABSTRACT: After different sports injuries, athletes may experience various psychological emotions in response to such injuries, which could lead an athlete to feel stressed. These emotions include anger, fear, frustration, anxiety, and depression which may lead to lack of confidence in returning to their sport and/or fear of sustaining a new injury. This narrative review aims to determine the possible psychological hindrances present when an athlete is planning on returning to sport after injury to an anterior cruciate ligament (ACL) or after sustaining a concussion. The synthesized information for this review has been collected from researching the databases PubMed, SportDiscus, PsycInfo, and Google Scholar using search terms including “return to sport”, “ACL injury”, “concussion”, and “psychology”. Journal articles needed to be in English and published in the years 2009-2019; books and unpublished abstracts were excluded. A total of 42studies were included and analyzed using deductive coding to organize and synthesize relevant articles into themes. The review summarizes the shared common and the different psychological hindrances that may be found in athletes after an ACL injury or concussion. Shared psychological characteristics for returning to sport following either an ACL injury or concussion included fear, self-esteem, control, anxiety, stress, recovery, and social support. Discovering the common and unique psychological barriers which may affect the injured athletes from returning to sport can help educate athletes’ families, coaches, and healthcare professionals, as well as promote discussions for the future to help athletes feel more secure in their return to their respective sport.
KEYWORDS: ACL Injuries; Concussions; Sport Injuries; Athletes; Narrative Literature Review; Psychological Hindrances; Psychological Characteristics; Return to Sport; Psychology

Click on this link to download the full high-definition interactive pdf for AJUR Volume 18 Issue 1 (June 2021)

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Links to individual manuscripts, abstracts, and keywords are provided below.

p3. Enzymatic and Structural Characterization of Alanine Racemase from Enterococcus faecium by Kinetic and Computational Studies
Arie Van Wieren^*, Emma Bouchard, & Sudipta Majumdar
https://doi.org/10.33697/ajur.2020.039
ABSTRACT: The surge in vancomycin-resistant enterococci (VRE)strains poses a serious threat to public and clinical health. VRE strains are the leading cause of multi-drug resistant enterococcal infections and are commonly acquired from medical devices. Therefore, it is essential to discover new antibacterial targets and drugs for this pathogen. Alanine racemase could be a valuable drug target due to its crucial role in E. faecium survival. Alr from E. faecium (EF_Alr) was heterologously produced and purified from E. coli., and the steady-state kinetic constants were determined at different pH values. Using a coupled reaction with L-alanine dehydrogenase, rate of production of NADH was measured at 340 nm to observe EF_Alr activity in the D- to L-alanine direction. The highest catalytic efficiency, 8.61 ± 0.5 s^-1 mM^-1, was found at pH 9. Additionally, the tentative active site residues, Lys40 and Tyr268, for the alanine racemization reaction were assigned by homology modeling and sequence comparison studies. Using UCSF Chimera, the structure of the EF_Alr homology model was superimposed and compared to the crystal structure of Alr from E. faecalis.
KEYWORDS: Alanine Racemase; Enterococcus faecium; Vancomycin-Resistant Enterococci; Homology Modeling; pH Optimum; Kinetic Characterization; SWISS-MODEL Server; Steady-State Kinetics

p.13 Is Play Sexually Dimorphic in the Polygamous Squirrel Monkey?
Seta Aghababian, Anita Stone, & Christopher Brown
https://doi.org/10.33697/ajur.2020.040
ABSTRACT: Play behavior is widespread in juvenile mammals and may be a mechanism for practicing skills needed in adulthood. In mammals characterized by strong adult male competition over females, juvenile males perform more social play than do females, and such play may assist in later mating competition. This study examined whether social play behavior is sexually dimorphic in a polygamous neotropical primate, the squirrel monkey (Saimiri collinsi), through a six-week field study of two groups of wild monkeys in Eastern Amazonia, Brazil. We hypothesized that males would conduct more rough-and-tumble play than females and that any sex-based play differences would be more evident in older juveniles. We video recorded juvenile play bouts and scored: age category (younger or older juvenile) and sex of players (male or female); and rough-and-tumble play behaviors (i.e., bite, grab, and wrestle). Juvenile males initiated more play bouts than did females. Most players were older juvenile males, while older juvenile females were the least represented. Older juvenile play bouts occurred mostly among males, while younger juvenile bouts consisted of a more even sex distribution. While younger juveniles did not significantly affect the number of rough-and-tumble behaviors in bouts, the number of behaviors was significantly affected by the sex of older individuals. These results indicate that social play is sexually dimorphic in juvenile S. collinsi; specifically, males play more than females and sex differences are more pronounced in older cohorts.
KEYWORDS: Squirrel Monkeys; Mating System; Sexual Dimorphism; Juvenile Period; Development; Play Behavior; Social Behavior; Ethology

 

 

Click on this link to download the full high-definition interactive pdf for AJUR Volume 7 Issue 4 (March 2021)

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Links to individual manuscripts, abstracts, and keywords are provided below.

p.3. Factors Controlling Coral Skeletal U/Ca Ratios with Implications for their Use as a Proxy for Past Ocean Conditions
Emily Patterson, Spencer Eanes, Penelope Lancrete, Anne Gothmann*^a, & Paul Roback^b
aDepartment of Environmental Studies and Physics, St. Olaf College, Northfield, MN
^bDepartment of Mathematics, Statistics, and Computer Science, St. Olaf College, Northfield, MN
https://doi.org/10.33697/ajur.2020.031
ABSTRACT: Seawater temperature, salinity and carbonate chemistry have been shown to influence the uranium/calcium (U/Ca) ratios of scleractinian coral skeletons. This apparent sensitivity of U/Ca to multiple environmental parameters calls into question whether there is one environmental variable that most strongly controls coral U/Ca, and whether U/Ca can be straightforwardly applied as a paleoenvironmental proxy due to the tendency of environmental variables to covary in space and time. In this study, uranium concentration data from an existing compilation of tropical scleractinian coral U-series measurements is paired with environmental data from the World Ocean Atlas (WOA) and the Global Ocean Data Analysis Project (GLODAP) to examine the sensitivity of coral skeletal U/Ca to multiple seawater properties including temperature, salinity, pH, and saturation state. First, univariate linear regressions and multiple linear regressions were used to compare relationships between uranium and environmental parameters in the dataset with relationships observed in previous studies. Next, principal component analysis and regularized regression were used to identify the most likely predictors of coral U/Ca in order to create a multiple linear regression model. Results indicate that pH, Ω, alkalinity, and temperature are all significant predictors of uranium concentrations in coral. The magnitude and strength of relationships between U/Ca and environmental variables also differ across different genera. Seawater properties with strong correlations and small ranges make interpretation of these results difficult. However, results of these analyses indicate that U/Ca is dependent on multiple environmental parameters and that previously developed univariate regressions may be insufficient to characterize the full range of variables that influence coral [²³⁸U].
KEYWORDS: Coral; Paleoceanography; Proxy Calibration; Uranium; Multiple Linear Regression; Regularized Regression; Environmental Change; Oceanographic Databases

p.19. Analyzing Trends in Water Table Elevations at the Marcell Experimental Forest, Minnesota, U.S.A.
Anna Stockstad^*a, Ella Gray,^a Stephen Sebestyen^b, Nina Lany^c, Randall Kolka^b, & Marcella Windmuller-Campione^a
aDepartment of Forest Resources, University of Minnesota- Twin Cities, St. Paul, Minnesota
^bUSDA Forest Service Northern Research Station, Grand Rapids, Minnesota
^cUSDA Forest Service Northern Research Station, Durham, New Hampshire
https://doi.org/10.33697/ajur.2020.032
ABSTRACT: Water table fluctuations in peatlands are closely coupled with the local climate setting and drive critical ecosystem processes such as nutrient cycling. In Minnesota, USA, peatlands cover ten percent of the surface area, approximately 2.5 million hectares, some of which are actively managed for forest products. To explore the relationship between peatland water tables and precipitation, long-term data (1961 to 2019) were used from the Marcell Experimental Forest in northern Minnesota. Starting in 1961, water table data from seven peatlands, including two types of peatlands (bogs and fens), were measured. We used the Theil-Sen estimator to test for monotonic trends in mean monthly water table elevations for individual peatlands and monthly precipitation. Water levels in bogs were both more variable and had mean water table elevations that were closer to the surface. Individual trends of water table elevations differed among peatlands. Water table elevations increased over time in three of the bogs studied and decreased over time in two of the bogs studied. Trends within fens were notably nonlinear across time. No significant linear trend was found for mean monthly precipitation between 1961 and 2019. These results highlight differences in peatlands types, local physiography, and the importance of understanding how changes in long-term dynamics coupled with changing current conditions will influence the effects of water table fluctuations on ecosystem services. The variability of water table elevations in bogs poses potential difficulties in modeling these ecosystems or creating adaptive management plans.
KEYWORDS: Peatlands; Hydrology; Water tables; Bogs; Fens; Monitoring; Minnesota; Climate Change

p.33. Treatment Outcomes in a Partial Hospital Program for Patients with Social Anxiety Disorder: The Effects of Comorbid Major Depression
Allison Graham^a,b*, Douglas R. Terrill^a, Simone I. Boyd^a, Isabel Benjamin^a, Madeline Ward^a, & Mark Zimmerman^a,c
aRhode Island Hospital Department of Psychiatry, Providence, RI
^bDepartment of Cognitive, Linguistic, and Psychological Sciences, Brown University, Providence, RI
^cDepartment of Psychiatry and Human Behavior, Brown Alpert Medical School, Providence, RI
https://doi.org/10.33697/ajur.2020.033
ABSTRACT: Although previous studies have examined the impact of comorbid major depressive disorder (MDD) on social anxiety disorder (SAD), the results have been somewhat mixed. Furthermore, most studies have been conducted in inpatient or outpatient settings. Given the large body of literature that suggests that this particular comorbidity can have negative effects on treatment efficacy and outcomes, it is important to continue to explore its impact. The present study aims to clarify contradictions in the literature and expands on previous studies by examining patients in a partial hospitalization setting. Patients at Rhode Island Hospital with a diagnosis of SAD were compared to those with comorbid SAD and MDD on pre-treatment and post-treatment measures of anxiety and depression. The results indicated that while the comorbid group showed significantly less improvement post-treatment on anxiety symptoms and constructs related to remission from depression, they did not show significantly less improvement on depression symptoms. The implications of these results for clinical practice are discussed.
KEYWORDS: Social Anxiety Disorder; Major Depressive Disorder; Comorbid Anxiety Disorders; Comorbid Mood Disorders; Treatment Outcomes; Partial Hospitalization Program

p.41. Evaluation of Physical Activity Participation, Self-Efficacy and Outcome Expectancy for Employees Participating in Exercise Is Medicine® On Campus Program
Maximilian Gastelum-Morales*, Lisa J. Leininger ͣ, Joanna L. Morrisseyᵇ, Ryan Luke ͣ, & Mark DeBeliso^c
ͣ Department of Kinesiology, California State University, Monterey Bay, Marina, CA
ᵇDepartment of Psychology, University of Wisconsin, Green Bay, Green Bay, WI
^cDepartment of Kinesiology and Outdoor Recreation, Southern Utah University, Cedar City, UT, United States
https://doi.org/10.33697/ajur.2020.034
ABSTRACT: Exercise Is Medicine® On Campus (EIM-OC) is a worldwide initiative from the American College of Sports Medicine (ACSM) to promote physical activity (PA) at universities. California State University, Monterey Bay (CSUMB) implemented this initiative in Fall 2019 with offerings to students and employees. For employees, an “Introduction to Resistance Training Class” was offered. Participants attended classes two times per week, with the sessions lasting approximately fifty minutes. The purpose of this study was to evaluate the EIM-OC employee Introduction to Resistance Training class for its effectiveness on increasing PA, self-efficacy, and outcome expectancy. The research design was pre-post, with participants completing online questionnaires before and after the course. The Godin Leisure Time Physical Activity Questionnaire (LTPQ), Resistance Training Self-Efficacy and Outcome Expectancy Questionnaire, and Self-Efficacy and the Maintenance of Exercise Participation in Older Adults Questionnaire were included. The training class had a total of 14 female participants, 12 of which completed the pre- and post-questionnaires.There was a significant increase (t=-3.2, df=11, p=.004) in resistance training self-efficacy score following the course (M=3.52±1.03 versus M=4.31±.56). Resistance training outcome expectancy score was also statistically significant (t=-2.54, df=11, p=.01) following the course (M=4.48±.53 versus M=4.71±.37). There were increases in strenuous exercise days, physical activity scores, and future resistance training self-efficacy, although they were not statistically significant. The results of this study indicate that employee exercise classes, as part of the EIM-OC initiative, can be effective in increasing resistance training self-efficacy, and outcome expectancy. These indicators are important for individuals to maintain lifelong PA therefore future programming and research on EIM-OC should continue.
KEYWORDS: Exercise Is MedicineⓇ-On Campus; Resistance Training; Physical Activity; Exercise; Worksite Health Promotion Program; Self-Efficacy; Outcome Expectancy; Employees; California State University, Monterey Bay

p.49. Axisymmetric Thermal Finite Element Analysis of Effects of Intraocular Projector in the Human Eye
John A. Stark^*a, Craig D. Foster^b, & Charles Yu^c
aDepartment of Civil and Materials Engineering, University of Illinois at Chicago, IL
^bDepartment of Civil and Materials Engineering, University of Illinois at Chicago, IL
^cDepartment of Ophthalmology, Stanford University, CA
https://doi.org/10.33697/ajur.2020.035
ABSTRACT: Millions of people worldwide live with corneal opacity which continues to be one of the leading causes of blindness. Corneal opacity is treatable. However, the surgical methods for treating this condition, such as corneal transplantation and keratoprosthesis, have many complications. The use of an intraocular projector is a promising approach to treat corneal blindness. Like any device using electrical power, an intraocular projection device produces heat, which could potentially damage eye tissue. Australian and international standards state that there cannot be an increase of temperature of 2 °C caused by an implanted device. In order to determine if these standards are met, a 2D axisymmetric thermal analysis of the projector in the human eye is conducted in ANSYS Workbench. With the projector operating at its maximum wattage, our analysis shows that an air gap extension within the projector will help maintain the temperature increase below 2 °C.
KEYWORDS: Finite Element Analysis; Eye; Heat Dissipation; Axisymmetric; Thermal Conductivity; Internal Heat Generation; Corneal Blindness; ANSYS; Intraocular Projector

p.59. Prime Factors and Divisibility of Sums of Powers of Fibonacci and Lucas Numbers
Spirit Karcher, Mariah Michael
Department of Mathematics, Christopher Newport University, Newport News, VA
https://doi.org/10.33697/ajur.2020.036
ABSTRACT: The Fibonacci sequence, whose first terms are f0; 1; 1; 2; 3; 5; : : :g, is generated using the recursive formula Fn+2 = Fn+1 + Fn with F0 = 0 and F1 = 1. This sequence is one of the most famous integer sequences because of its fascinating mathematical properties and connections with other fields such as biology, art, and music. Closely related to the Fibonacci sequence is the Lucas sequence. The Lucas sequence, whose first terms are f2; 1; 3; 4; 7; 11; : : :g, is generated using the recursive formula Ln+2 = Ln+1 + Ln with L0 = 2 and L1 = 1. In this paper, patterns in the prime factors of sums of powers of Fibonacci and Lucas numbers are examined. For example, F2 3n+4 + F2 3n+2 is even for all n 2 N0. To prove these results, techniques from modular arithmetic and facts about the divisibility of Fibonacci and Lucas numbers are utilized.
KEYWORDS: Fibonacci Sequence; Lucas Sequence; Modular Arithmetic; Divisibility Sequence

p.71. A Survey of Inhibitors for the Main Protease of Coronaviruses with the Potential for Development of Broad-Spectrum Therapeutics
Alyssa Sanders^*a, Samuel Ricci^b, Sarah Uribe^a, Bridget Boyle^a, Brian Nepper^b, & Nathaniel Nucci^a,b
aDepartment of Molecular & Cellular Biosciences, Rowan University, Glassboro, NJ
^bDepartment of Physics & Astronomy, Rowan University, Glassboro, NJ
https://doi.org/10.33697/ajur.2020.037
ABSTRACT: The coronaviruses plaguing humanity in the 21st century share much in common: a spontaneous route of origin from wild animals, a propensity to take human life, and, importantly, a highly conserved set of biological machinery necessary for viral replication. Most recently, the SARS-CoV-2 is decimating economies around the world and has claimed over two million human lives, reminding the world of a need for an effective drug against present and future coronaviruses. To date, attempts to repurpose clinically approved antiviral medications show minimal promise, highlighting the need for development of new antiviral drugs. Nucleotide analog inhibitors are a promising therapeutic candidate, but early data from clinical studies suggests these compounds have limited efficacy. However, novel compounds targeting the main protease responsible for critical steps in viral assembly are gaining considerable interest because they offer the potential for broad-spectrum coronavirus therapy. Here, we review the literature regarding potential inhibitors for the main protease of coronaviruses, especially SARS-CoV-2, analyze receptor-drug interactions, and draw conclusions about candidate inhibitors for future outbreaks. Promising candidates for development of a broad-spectrum coronavirus protease inhibitor include the neuraminidase inhibitor 3K, the peptidomimetic inhibitor 11a and 11b, the α-ketoamide inhibitor 13b, the aldehyde prodrug, and the phosphate prodrug developed by Pfizer. In silico and in vitro analyses have shown that these inhibitors strongly interact with the active site of the main protease, and to varying degrees, prevent viral replication via interactions with the largely conserved active site pockets.
KEYWORDS: Severe Acute Respiratory Syndrome Coronavirus; Middle East Respiratory Syndrome Coronavirus; Severe Acute Respiratory Syndrome Coronavirus 2; Replicase Polypeptide; Protease; Neuraminidase Inhibitor; Peptidomimetic Inhibitor; α-Ketoamide Inhibitor; Molecular Docking

 

Click on this link to download the full high-definition interactive pdf for AJUR Volume 7 Issue 3 (December 2020)

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Links to individual manuscripts, abstracts, and keywords are provided below.

p.3 Investigating the Effect of Flock Size on Vigilance in the American Coot (Fulica americana) in Relationship to Habitat
Dat Q. Lam, Suyash P. Rizal, Roxanne Cota, Miguel Sicaja, Gabriel Cox, Brandon Wakefield, & Zia Nisani
Department of Biological and Environmental Sciences, Antelope Valley College, Lancaster, CA
https://doi.org/10.33697/ajur.2020.022
ABSTRACT: Among many anti-predator behaviors, vigilance is observed in many species and plays an important role in survival. In this study, we investigated the effect of flock size on vigilance in American Coots (Fulica americana)foraging on land and water, by observing individual birds in these habitats and recording the time spent scanning (i.e., vigilance). Mean flock size was larger on land compared to water and vigilance negatively correlated with flock size. Birds in water were more vigilant compared to on land, regardless of whether they were foraging alone or in flocks. However, the effect of flock size on vigilance showed a weak linear correlation as it was possible that other factors (e.g., human habituation, food kleptoparasitism, or scramble competition) could have also played a role in shaping vigilance. These results suggest that there is a relationship between flock size and vigilance, which are related to previous researches that show a negative correlation between vigilance and flock size.
KEYWORDS: Birds; American Coot; Vigilance; Scanning; Foraging; Flock Size; Habituation; Competition; Behavior

p.11 Cyclophosphamide Depletes Ovarian Follicles in Mice During Both the Light and Dark Phases of the Circadian Cycle
Benjamin Z. Koch & Kristen A. Roosa^*
Biology Department, State University of New York College at Oneonta, Oneonta, NY
https://doi.org/10.33697/ajur.2020.023
ABSTRACT: The alkylating agent cyclophosphamide (CY) is a potent ovarian toxicant. It damages growing follicles and causes premature activation and depletion of the resting follicles that constitute the ovarian reserve. While there is abundant information on the impact of CY on the ovary and its toxicity mechanisms, the influence of the circadian rhythm on ovarian toxicity has not been evaluated. To test the hypothesis that time of exposure affects ovarian toxicity of CY, C57BL/6 mice were treated with a single injection of CY (75 mg/kg) at either two hours after lights on (Zeitgeber time (ZT) 02) or two hours after lights off (ZT14). Toxicity was evaluated one week after treatment by counting ovarian follicles in histological sections. Fewer primordial follicles were counted in the ovaries of CY-treated animals at both treatment times, and fewer antral follicles were counted in the ovaries of animals treated at ZT02. There was no difference in the number of primordial follicles in the ovaries of CY-treated animals between the two treatment times. These results demonstrate that CY-induced depletion of the ovarian reserve occurs when mice are exposed early in the light phase and early in the circadian cycle’s dark phase. There is no impact of the circadian rhythm on follicle depletion by CY at these time points.
KEYWORDS: Cyclophosphamide; ovary; circadian; ovarian follicles; toxicity; mouse; chronotherapy; alkylating agent

p.19 Associations of Maternal Controlling Feeding Practices with Child Internalizing Symptoms and Body Mass Index in Ethnically-Diverse Mother-Child Dyads
Paulina Mozdzierz, Genevieve F. Dunton, & Tyler B. Mason*
Department of Preventive Medicine, University of Southern California, Los Angeles, CA
https://doi.org/10.33697/ajur.2020.024
ABSTRACT: Mothers may use controlling feeding practices (i.e., pressure to eat and restriction) to regulate their child’s weight. However, these practices may have unintended consequences on the weight and mental health of children. The first aim of this study was to investigate differences in maternal controlling feeding practices by child gender, age, and maternal ethnicity. The second aim was to examine cross-sectional associations among maternal controlling feeding practices, child body mass index z-scores (BMI-z), global internalizing symptoms (i.e., depression and anxiety symptoms), and self-esteem. The third aim was to determine whether child sex and mother ethnicity moderate these associations. A sample of 202 ethnically diverse mother-child dyads (children ages 8-12; 49% female) completed self-report questionnaires and had weight and height measurements taken. Results showed no differences in maternal controlling feeding practices by gender, ethnicity, or age. Pressure to eat was negatively related to child BMI-z, and restriction was positively related to BMI-z. Moreover, pressure to eat was negatively related to child self-esteem. There were no associations between maternal controlling feeding practices and global internalizing symptoms. Further, no associations differed by child gender or mother ethnicity. Maternal controlling feeding practices may be used to move a child’s weight toward a healthy weight range. Overall, there was little evidence for associations between feeding practices and poor mental health; although, pressure to eat was related to poorer self-esteem in children.
KEYWORDS: Maternal; Feeding; Practices; Child; BMI-z; Mental; Health; Controlling; Restricting

p.29 Synthesis of Graphene Oxide Enhanced Agar Composites: A Biocompatible Photo-catalyst for Degradation of Organic Dyes
Shreyas Dindorkar*^a, Jaymin Mistry^a, Jayesh Hire^a, Khushi Jain^b, Nandini Khona^b, Shreya Peddakolmi^b, & Paresh More^ab
a Department of Chemistry, K. E. T’s, Vinayak Ganesh Vaze College (Autonomous), Mulund, Mumbai, India
^b Department of Biotechnology, K. E. T’s, Vinayak Ganesh Vaze College (Autonomous), Mulund, Mumbai, India
https://doi.org/10.33697/ajur.2020.025
Abstract: Herein we report the synthesis of graphene oxide-based agar composites using a solution casting method. Graphene oxide was synthesized by modified Hummer’s method and was characterized using X-ray diffraction (XRD) and Raman spectroscopy. The graphene oxide-based agar composites were characterized using X-ray diffraction (XRD) and UV-visible spectroscopy. Optical band gap obtained from the Tauc plot showed that the composites could be used in the photodegradation of dyes. The synthesized composite material was checked for its practical applicability in the degradation of methylene blue dye under solar irradiation; with an increase in the concentration of graphene oxide, catalyst, and H₂O₂, the rate constant increases. The rate constant was found to be inversely proportional to the concentration of methylene blue dye. Dosage of graphene oxide was found to be the most prominent factor in increasing the rate of photodegradation. It is clear from the data for the reaction system that the degradation reaction follows pseudo-first-order kinetics.
Keywords: Composites; Ultra-sonication; Photodegradation; Methylene Blue; XRD; Graphene Oxide; Kinetics; Biocompatibility

p.41 The Evolution of Multidrug Resistance in an Isolated Pseudomonas Strain
Allison Grodnick*^a, Ashley Fink^a, Timothy J. Johnson^bc, & David Mitchell^a
aDepartment of Biology, College of St. Benedict St. John’s University, Collegeville, MN
^bDepartment of Veterinary and Biomedical Sciences, University of Minnesota, St. Paul, MN
^cMid-Central Research and Outreach Center, University of Minnesota, Willmar, MN
https://doi.org/10.33697/ajur.2020.026
ABSTRACT: As an unintentional result of the extensive use of antibiotics in healthcare and agriculture, antibiotics have become an increasingly prevalent selective pressure on bacteria. This forces bacteria to evolve and acquire antibiotic-resistant genes or mutations in order to survive. Suppose a bacterial strain acquires resistance to three or more antibiotics. In that case, it is deemed multidrug-resistant (MDR), and it becomes a potentially more serious problem to solve in the context of healthcare. This study aims to evaluate the acquisition of resistance to multiple antibiotic drugs by an initially susceptible isolated bacterium from a Minnesota forest environment. The bacterium was found to be Pseudomonas by 16s rRNA gene sequencing. Three antibiotics, neomycin, ciprofloxacin, and imipenem, each from a different drug class, were selected to see if this isolate could become resistant over time and exposure. The bacterial strain developed resistance to the selected antibiotics through a series of sequential exposures to increasing concentrations of each drug in this order. As determined by a disc susceptibility test, the initial isolate acquired resistance to all three selected antibiotics. Single nucleotide polymorphisms (SNPs) between the original isolate and the final resistant strain were identified. These SNPs suggest that mutations to efflux transporters and antibiotic protein targets play a role in acquiring and maintaining antibiotic resistance.
KEYWORDS: Multidrug Resistance; Antibiotics; Neomycin; Ciprofloxacin; Imipenem; Pseudomonas; Evolution; MDR; Minnesota Environment

p.51 Stripping Material from a Supported Lipid Bilayer with High Speed Buffer Flow
Michael J. Ornstead, Ruth Hunter, Mason L. Valentine, Cameron Cooper, Stephen K. Smith, & Christopher F. Monson*
Department of Physical Science, Southern Utah University, Cedar City, UT
https://doi.org/10.33697/ajur.2020.027
ABSTRACT: A microfluidic device was created and used to demonstrate that supported lipid bilayers can be deposited on clean glass slides and removed using high velocity buffer flow (1-4 m/s linear velocity). This was accomplished by forcing the flow through a microfluidic channel covering an annealed glass coverslip bearing a supported lipid bilayer (SLB). The removal of bilayer material was monitored via fluorescence microscopy, and two basic regimes were observed: at 1-2 m/s smaller areas were stripped, while at 3-4 m/s larger areas were stripped. SLB removal was verified by two means. First, lipid vesicles labeled with a different fluorescent dye were added to the device and filled in holes left by the removal of the original SLB, allowing stripping to be verified visually. Second, the solutions obtained from stripping were concentrated and the fluorescence in the concentrates was measured. The ability to strip SLB from glass provides a relatively gentle method of creating spatially inhomogeneous SLB, which could be a useful tool in the continued investigation of membrane properties and components.
KEYWORDS: Supported Lipid Bilayer; Membrane Vesicle; Microfluidic Device

p.61 Assessing Initiatives for Rural Health Practices in South Carolina
Aalia Soherwardy*^a & Elizabeth Crouch^b
aUniversity of South Carolina School of Medicine, Columbia, SC
^bRural and Minority Health Research Center, Arnold School of Public Health, University of South Carolina, Columbia, South Carolina
https://doi.org/10.33697/ajur.2020.028
ABSTRACT: The purpose of this study was to determine which incentives are most effective in motivating medical students to practice in rural areas of South Carolina, which can be informative for the medical practitioner rural recruitment process. Medical students attending the University of South Carolina School of Medicine located in Columbia, South Carolina were surveyed about demographic information, motivations for rural practice, and considerations for choosing a practice location (n=109). Chi-square tests and bivariate analyses were used to test for significant differences. A significant relationship was found between previous residence in a rural area and personal motivation to practice in a rural area (p<0.001). It was also found that 86.2% of students who had previously lived, worked, or served in rural areas had a personal motivation to practice medicine in a rural area, confirming previous research. Loan forgiveness options were the most appealing personal incentive for the students in this study, closely followed by guaranteed minimum incomes and tax incentives; financial incentives were more preferred than non-financial incentives like reduced on-call work and accelerated residencies. The results of this study can be utilized to craft future state-supported incentive programs or to tailor current programs to more effectively recruit students to rural practice.
KEYWORDS: Rural; Recruitment; Healthcare Provider; Shortage; Incentive Programs; Medical Student; Southern United States; Loan Forgiveness

p.73 Travel Through Time: From 9/11 to COVID-19, Parallel Predictive Analysis of Travel Marketing
Hannah Gilliam^*
Gainey School of Business, Spring Arbor University, Spring Arbor, MI
https://doi.org/10.33697/ajur.2020.029
ABSTRACT: The events of 9/11 drastically changed the state of the nation across many industry sectors, with the tourism industry among those most affected. Following that horrific day, the nation experienced heightened security measures and protocol, such that the travel industry and travelers would never look the same. People were fearful and anxious, and the tourism industry had to take quick, effective measures to evaluate the consumer response, set a marketing strategy, and promote within a changed national ethos and expectations. COVID-19 is a similar catastrophic, global, and long-term crisis that set our nation on a similarly drastic change in practice and protocol; fear and anxiety were higher than ever. COVID-19 and 9/11 are highly comparable in their market response. By comparing the two events and analyzing the consumer response and advertising messaging, specifically during the stay at home order, a theme and direction for messaging within the travel industry post-COVID-19 can be predicted based on the culture and spirit of The American Dream, confidence in safety, we are in this together, support local tourism, explore your city in a new way, and connect with those you missed.
KEYWORDS: COVID-19; 9/11; Post-pandemic; Advertising; Travel; Prediction; Messaging; Consumer Response; Marketing; Analysis

Click on this link to download the full high-definition interactive pdf for AJUR Volume 7 Issue 2 (September 2020)

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Links to individual manuscripts, abstracts, and keywords are provided below.

p.3. Bacillus cereus & Bacillus pumilus Harvested from a Copper Roof Inhibit the Growth of Other Microorganisms
Alison Stiller, Ashley Fink, & David Mitchell
https://doi.org/10.33697/ajur.2020.016
ABSTRACT: Bacteria growing under the effects of unique selective pressures have distinct adaptations allowing them to survive. Copper surfaces present challenges for bacterial survival because ions dissolve from the surfaces and disrupt cell membranes, thus inhibiting bacterial growth. In this study, the copper roof of Simons Hall in Collegeville, Minnesota was sampled for bacterial species during November 2018. Bacteria were isolated and grown in culture, and zones of inhibition were identified surrounding three of the bacterial colonies. Polymerase chain reaction (PCR) was used to identify two of the bacteria samples as Bacillus cereus and a third sample as Bacillus pumilus. Bacilli are large, rod-shaped, gram-positive bacteria commonly found in diverse environments. They are endospore-forming aerobes or facultative anaerobes. Initial experiments indicated that all three Bacillus strains had the ability to inhibit the growth of three environmental microorganisms. Results from growth curve experiments depicted inhibitory effects on environmental microorganisms at all stages of the growth curve, which is contrary to the prediction that the inhibitory behavior would appear at one specific period of the growth curve. Additional experiments involved plating isolates of Bacillus cereus and Bacillus pumilus with laboratory samples of Pseudomonas aeruginosa, Streptococcus pneumoniae, and Listeria monocytogenes to further understand the effectiveness of B. cereus and B. pumilus at inhibiting the growth of other microorganisms. These findings support previous studies and suggest that Bacillus are capable of inhibiting or killing other organisms. Further research will be conducted to illuminate the inhibitory mechanisms and identify potential therapeutic possibilities.
KEYWORDS: Bacteria; Copper; Resistance; Growth Curve; Inhibition; Bacillus; Bacteriocin; Antimicrobial Peptides

p. 13. An Assessment of Sleep Duration and Determinants of Health in a Cross-Sectional Sample of Gynecologic Cancer Survivors in Los Angeles County
Ma’at Hembrick, Makala E. Conner, & Heather P. Tarleton
https://doi.org/10.33697/ajur.2020.017
ABSTRACT: Cancer survivors have an increased risk of treatment-related deficits in physical health and low health-related quality of life. In this cross-sectional study, a health questionnaire was mailed to women from the Los Angeles County Cancer Surveillance Program aged 45-70 and diagnosed with cervical, endometrial, or ovarian cancer in 2005-2014. Of the 5,941 surveys with valid postal addresses, 586 (10%) were completed and returned. The average age of respondents was 66 years old, and 36% identified as non-white. Non-white respondents were less likely to have a college degree (p<0.001), more likely to sleep for less than seven hours each night (p<0.001), experience bodily pain (p<0.001), and have a diagnosis of cervical cancer (p=0.002), when compared to white respondents. Health behaviors and determinants were examined across cervical, endometrial, and ovarian cancer cases. Cervical cancer survivors reported sleeping less than 7 hours per night, on average (p=0.015). Race was associated with sleep duration among endometrial (p=0.002) and ovarian (p=0.003) cancer survivors. Menopausal status was associated with the relationship between race and sleep duration (p<0.001). Depression was inversely related to sleep duration (p = 0.022) but was not associated with race, menopausal status, time since treatment, physical activity, or cancer type. Postmenopausal cervical cancer survivors reported a moderate concern about fall risk compared to their premenopausal counterparts (p=0.048). Physical activity levels increased as time since treatment increased (p=0.003) regardless of cancer type. Race, menopausal status, depression, and cancer type impacted the sleep duration.
KEYWORDS: Health Disparities; Sleep Duration; Depression; Gynecologic Cancers; Survivorship Care

p.27. An Unlikely Symbiosis: Science and Law
Evan Miller
https://doi.org/10.33697/ajur.2020.018
ABSTRACT: Science has historically held a position of high regard in society. Science is intimately connected to law. These disciplines meet in the courtroom. Due to the nature of civil and criminal disputes in the United States, litigators retain expert witnesses to explicate nuanced subjects, including science. Unfortunately, the common law system has not always favored sound science. This paper examines how science and law can work in concert to benefit all people. Some feel that scientists should simply educate courtrooms, but further scrutiny questions the feasibility of this approach. Understanding the sociology of scientific knowledge elucidates this debate and is applied to the forensic sciences. Science and law have the capacity to improve the human condition and increase equity among all people.
KEYWORDS: Science Communication; Expert Witnesses; Science; Public Perception; Law; Misinformation

p.35. Microfibers in Mytilus species(Mollusca, Bivalvia)from Southern California Harbors, Beaches, and Supermarkets
Chloe Mankin & Andrea Huvard
https://doi.org/10.33697/ajur.2020.019
ABSTRACT: Plastic microfibers are an emerging threat to terrestrial and aquatic habitats worldwide. They are equivalent in size to planktonic organisms, making them available to a range of invertebrates. Bivalve mollusks can ingest and accumulate plastics via digestion and adherence to soft tissue. We determined the frequency and characteristics of microfiber pollution from wild Mytilus californianus and farmed Mytilus edulis populations that were collected from southern California harbors, beaches, and supermarkets (n=4 per site). Mussel organic matter was dissected using 30% H₂O₂. After adding a saline solution to separate the fibers, the liquid was filtered by vacuum filtration. The remaining fibers were examined with a magnification of 1~8x. In this short-term survey, the abundance of microfibers by sample and by shell length showed a significantdifference between the harbor and beach sites. However, there was not a significant difference between the wild and farmed mussels examined. This study revealed widespread microfiber pollution and uptake by mussels in these locations and reinforces how bivalves can be used as a bioindicator of microfiber pollution.
KEYWORDS: Microplastic; Microfiber; Mytilus; Biomonitoring; Bivalvia; Seafood; Human Health

 p.45. A Brief History and Overview of Existential-Phenomenological Psychology
Christopher Zieske
https://doi.org/10.33697/ajur.2020.020
ABSTRACT: This article surveys the background and theory of the existential-phenomenological approach to psychology, with a particular focus on its reception in the United States. The article begins with a discussion of what exactly existential-phenomenological psychology is, including the theories underlying this approach and its basic practices. The article then discusses how the approach developed, including its roots in the philosophies of existentialism and phenomenology, its first appearances in Europe, its globalization, and finally its arrival in the U.S. The article then discusses struggles that the existential-phenomenological movement in psychology is currently facing and the concerns of those involved in the movement for its future. Finally, the article closes on a summary of all the information presented as well as of the contributions to the field of psychology that it and the existential-phenomenological movement can make.
KEYWORDS: Existentialism; Phenomenology; Psychology; United States; Existential Psychology; Phenomenological Psychology; Existential Psychotherapy; Philosophy of Psychology

Click on this link to download the full high-definition interactive pdf for AJUR Volume 7 Issue 1 (June 2020)

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Links to individual manuscripts, abstracts, and keywords are provided below.

p.3. 9-Borobicyclo[3.3.1]nonane-Catalyzed Hydroboration of Terminal Aromatic Alkynes with Pinacolborane
Garett L. Ruesch, Sydney L. Rowley, Marcus C. Mifflin, & Nathan S. Werner*
Department of Physical Science, Southern Utah University, Cedar City, UT
https://doi.org/10.33697/ajur.2020.011
ABSTRACT: Organoboron compounds are extensively used in organic synthesis. The alkenylboronic acid pinacol esters formed from the hydroboration reaction of alkynes with pinacolborane are stable, easy to handle, and useful in many synthetic transformations. However, pinacolborane lacks the reactivity necessary to undergo facile hydroboration reaction with terminal aromatic alkynes. 9-Borobicyclo[3.3.1]nonane (9-BBN) can be used to catalyze the hydroboration reaction of phenylacetylene with pinacolborane. The hydroboration reaction parameters and product purification conditions were evaluated to maximize the yield of (E)-2-phenylethenylboronic acid pinacol ester. It was found that the optimal reaction conditions for the 9-BBN-catalyzed hydroboration of phenylacetylene with pinacolborane were: phenylacetylene (1.0 equiv), pinacolborane (1.2 equiv), 9-BBN (20 mol%), and THF [0.2] at 65 °C. The compatibility of these reaction conditions with p-substituted terminal aromatic alkynes bearing electronically diverse groups was studied. Moderate to good yield (49–76%) of the hydroboration products were isolated after purification by liquid-liquid extraction and flash chromatography.
KEYWORDS: Organic Synthesis; Catalysis; Methods Development; Hydroboration; Reaction Optimization; Alkenylboronic Ester; Alkyne; Pinacolborane; 9-Borobicyclo[3.3.1]nonane

p.13. Synthetic Biology Bicistronic Designs Support Gene Expression Equally Well in vitro and in vivo
Owen Koucky^a, Jacob Wagner^b, Sofia Aguilera^b, Benjamin Bashaw^b, Queena Chen^a, Anthony Eckdahl^a, Elise Edman^c, Paul Gomez^a, Nick Hanlan^b, Nick Kempf^d, Devin Mattoon^d, Sam McKlin^a, Christopher Mazariegos^a, Alex Morehead^d, Shi Qing Ong^b, Andy Peterson^c, Maria Rojas^a, Kyla Roland^a,, Kaitlyn Schildknecht^c, Haley Seligmann^c, Kaden Slater^d, Ali Tauchen^b, Raechel Tittor^b, Tatianna Travieso^a, Dannie Urban^d, Caroline Willis^a, John Zhou^a, Nicole L. Snyder^e, Laurie J. Heyer^c, Jeffrey L. Poet^d, Todd T. Eckdahl^b, & A. Malcolm Campbell*^a
aDepartment of Biology, Davidson College, Davidson, NC
^bDepartment of Biology, Missouri Western State University, St. Joseph, MO
^cDepartment of Mathematics and Computer Science, Davidson College, Davidson, NC
^dDepartment of Computer Science, Math, and Physics, Missouri Western State University, St. Joseph, MO
^eDepartment of Chemistry, Davidson College, Davidson, NC
https://doi.org/10.33697/ajur.2020.012
ABSTRACT: Synthetic biology integrates molecular biology tools and an engineering mindset to address challenges in medicine, agriculture, bioremediation, and biomanufacturing. A persistent problem in synthetic biology has been designing genetic circuits that produce predictable levels of protein. In 2013, Mutalik and colleagues developed bicistronic designs (BCDs) that make protein production more predicable in bacterial cells (in vivo). With the growing interest in producing proteins outside of cells (in vitro), we wanted to know if BCDs would work as predictably in cell-free protein synthesis (CFPS) as they do in E. coli cells. We tested 20 BCDs in CFPS and found they performed very similarly in vitro and in vivo. As a step toward developing methods for protein production in artificial cells, we also tested 3 BCDs inside nanoliter-scaled microfluidic droplets. The BCDs worked well in the microfluidic droplets, but their relative protein production levels were not as predictable as expected. These results suggest that the conditions under which gene expression happens in droplets result in a different relationship between genetic control elements such as BCDs and protein production than exists in batch CFPS or in cells.
KEYWORDS: Bicistronic Design; Synthetic Biology; Cell-Free Protein Synthesis; Microfluidics
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Supplement 2

p.21. Survey of Wolbachia frequency in Nashville, Tennessee Reveals Novel Infections
Sangami Pugazenthi, Phoebe White, Aakash Basu, Anoop Chandrashekar, & J. Dylan Shropshire^*
Department of Biological Sciences, Vanderbilt University, Nashville, TN 37235, USA
https://doi.org/10.33697/ajur.2020.013
ABSTRACT: Wolbachia (Rickettsiales: Anaplasmataceae) are maternally transmitted intracellular bacteria that infect approximately half of all insect species. These bacteria commonly act as reproductive parasites or mutualists to enhance their transmission from mother to offspring, resulting in high prevalence among some species. Despite decades of research on Wolbachia’s global frequency, there are many arthropod families and geographic regions that have not been tested for Wolbachia. Here, arthropods were collected on the Vanderbilt University campus in Nashville, Tennessee, where Wolbachia frequency has not been previously studied. The dataset consists of 220 samples spanning 34 unique arthropod families collected on the Vanderbilt University campus. The majority of our samples were from the families Blattidae (Blattodea), Pulicidae (Siphonaptera), Dryinidae (Hymenoptera), Aphididae (Hemiptera), Paronellidae (Entomobryomorpha), Formicidae (Hymenoptera), Pseudococcidae (Hemiptera), Sphaeroceridae (Diptera), and Coccinellidae (Coleoptera). PCR-based techniques were used to assign infection states and, from these data, the first cases of Wolbachia in the Paronellidae springtails, Lithobiidae (Lithobiomorpha) centipedes, Lonchopteridae (Diptera) spear-winged flies, Sepsidae (Diptera) black scavenger flies, Cryptocercidae (Blattodea) wood roaches, and Lauxaniidae (Diptera) acalyptrate flies were identified. Within-family infection frequencies ranged from 17-100% when Wolbachia was observed; however, numerous families tested did not reveal evidence of infection. These results expand on the field’s understanding of Wolbachia’sfrequencyin Nashville, Tennessee, and among arthropod families broadly, and is the first report of Wolbachia in centipedes.
KEYWORDS: Wolbachia; Infection Frequency; Endosymbiont; Tennessee; Centipede; Arthropod; Polymerase Chain Reaction; Nashville